Medical microbiology Assignment Example | Topics and Well Written Essays - 750 words

Medical microbiology - Assignment Example around of them are insufficient media, disinfectant activity present in wrinkle, quantity of tide rip used for culturing, antibiotics occurrence, consanguinity collection time. Most of bacterial cells of Salmonella serovar Typhi resides intracellulary in the patients subscriber line.There is a need of reliable, fast and sensitive methods for clinical detection of Salmonella serovar Typhi. Serological tests are available but its sensitivity and specificity is very low. The business gloss sensitivity is highest during the first week of illness but it then gradually decreases with advancement of illness. 45- 70% patients of typhoid fever can be identified through blood culturing. It depends upon the blood sample amount, bacteraemic aim of Salmonella, culture medium type, length of incubation.Also the Salmonella bacteria straw man in patients blood is very low, often below detection by blood culturing and even below detection by PCR. D ifficulties become more due to limited amount of patient blood sample.Ox bile tryptone soja bean broth was used for blood culture it allows blood cell lysis completely, which causes the release of intracellular bacteria. It does not master the growth of Salmonella Typhi. From research it was found out that bacterial growth can be increased if blood culture media contains lyzing agents for blood cells. By performing various tests it was found out that 2.4% ox bile presence in blood culture will lyse blood cells within 1.5 hours so that the bacteria present intracellulary came out.Salmonella bacteria when kept in tryptone soya broth containing 2.4% ox bile for 3 hours will increase the amount of bacteria from 0.75 CFU per milliliter of blood. This level can be easily detectible by using regular PCR method. This blood culture PCR assay processing time is lesser than 8 hours as compared to conventional blood culture which may take 2 -5 days.Salmonella serovars patients blood or serum is also bactericidal